Other UTP ELISA Kit orb781964
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Catalog Number orb781964 Category Assays and Kits Description This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Uridine Triphosphate(UTP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Uridine Triphosphate(UTP). Tested applications ELISA Reactivity All Range 78.13-5000 ng/mL Concentration 5000 ng/mL Sensitivity 23.2 ng/mL Protein Length 2h Alternative names UTP
Read more...Note For research use only Application notes Standard: 5000 ng/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with UTP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to UTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UTP in the samples is then determined by comparing the OD of the samples to the standard curve Sample Types serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Expiration Date Please enquire.
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