Human ASMA(Anti-Smooth Muscle Antibody) ELISA Kit orb1146885 https://www.biorbyt.com/human-asmaanti-smooth-muscle-orb1146885.html
| Catalog Number | orb1146885 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Smooth Muscle Antibody(ASMA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Smooth Muscle Antibody(ASMA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Smooth Muscle Antibody(ASMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Smooth Muscle Antibody(ASMA) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 7.82-500 ng/mL |
| Sensitivity | 3.2 ng/mL |
| Protein Length | 3.5h |
| Alternative names | ASM Ab, Anti Smooth Muscle Antibody Read less |
| Note | For research use only |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Expiration Date | Please enquire. |