25-OH-D(25 Hydroxy Vitamin D) ELISA Kit orb1146945
https://www.biorbyt.com/25-oh-d25-hydroxy-vitamin-d-orb1146945.html
| Catalog Number | orb1146945 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25 Hydroxy Vitamin D(25-OH-D) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25 Hydroxy Vitamin D(25-OH-D). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25 Hydroxy Vitamin D(25-OH-D) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | All |
| Range | 3.13-200 ng/mL |
| Sensitivity | 0.91 ng/mL |
| Protein Length | 2h |
| Alternative names | 25 Hydroxy Vitamin D, Read more... |
| Note | For research use only |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |