荧光标记物如何选择 508播放 展开

【Biorbyt 技术】标记抗体到底好在哪？什么是标记抗体？标记抗体一般是指将荧光素或荧光团，生物素或者酶（HRP, AP）等标记物通过共价键或其他方式连接到一抗或二抗的特定部位上后所得到的直标抗体。➤ 荧光素标记荧光色素是最常用于标记抗体的标记物之一。荧光素经恰当的激发可发光，从而得知抗体的定位和待测抗原的分布情况，主要用于细胞分选或高分辨率免疫染色，是一种非常好的亚细胞水平精确定位的方法。➤ 酶标记酶标记抗体是将酶（HRP，AP等）与特异性抗体经适当方法连接而成，其应用范围非常广泛，结果即时可见、敏感性高，但较难应用于定量分析。➤ 生物素标记生物素（Biotin）标记反应简单、温和且很少抑制抗体活性，将生物素与抗体共价结合是一种非常简便、直接的标记方法。生物素与亲和素的反应体系可以扩大信号反应级别，有助于表达量低的蛋白检测。标记抗体的优势增强放大效应：标记物如酶、荧光素等具有高度的敏感性。荧光素或酶等标记物的分子量相对IgG来说较小，如一个IgG分子上可以标记15-20个FITC分子，一个IgG分子也可以标记多个HRP分子，通过这些标记物的增强放大效应，可以更直观地观察到抗原与抗体的反应情况，从而更准确地判断结果。提高检测灵敏度：通过标记技术，如酶标记法、荧光素标记法、生物素标记法等，抗体与标记物的结合能够形成多元复合物，这种复合物的形成不仅增强了检测的灵敏度，还提高了识别的特异性。这使得标记抗体在检测过程中能够更准确地识别和结合特定的抗原，从而提高检测的准确性。定位分析：标记抗体主要用于抗原的定位分析，特别是在细胞、亚细胞、超微结构及分子水平上对抗原、抗体反应进行深入的定性和定位研究。例如，荧光素标记的抗体可以用于高分辨率免疫染色，实现亚细胞水平的精确定位。定量检测：在某些情况下，标记抗体也可以对混杂有大量其他分子的样本中的抗原进行定量检测。例如ELISA试剂盒的开发中就用到了生物素标记的一抗，最终实现了ELISA试剂盒的定量检测。方便应用：例如荧光标记的一抗，在免疫荧光或流式细胞术实验时，在孵育完荧光一抗后，就可以直接显微镜观察，无需再加入二抗。标记抗体的应用1）免疫荧光染色：荧光一抗可用于组织或细胞的荧光染色。荧光一抗在免疫荧光实验中不仅省去了加二抗的过程，使实验更便捷。也更多用于多色免疫荧光共染中，因为商业化的一抗大多为兔抗或者鼠抗，当要进行多个靶标的多色荧光共染时，往往面临二抗选择问题，这时就可以选择不同颜色的荧光一抗。2）流式细胞术：荧光抗体技术结合流式细胞仪，可用于细胞表型和功能的快速分析，为生物医学研究和临床应用提供重要支持。3）ELISA：ELISA试剂盒的开发主要分为夹心法和竞争法，在试剂盒的开发中也会用到标记一抗，主要是生物素标记的一抗，一般一个IgG分子量可以结合3-5个生物素分子，然后通过生物素 与 亲和素的特异性结合从而起到放大效应。另外，利用FITC与anti-FITC结合放大效应，FITC标记的一抗也可用于试剂盒的开发中。4）其他应用：如酶标二抗和荧光二抗。在WB，IHC，ELISA等实验中，一般都要用到酶标二抗，而在免疫荧光或流式细胞术中，则需要用到荧光二抗。常用荧光标记物FITC：异硫氰酸荧光素（Fluorescein Isothiocyanate），是一种对pH和 Cu2+敏感的荧光染料。可用作胺标记的荧光探针，CAS 3326-32-7。分子量为389.4，最大吸收光波长为490～495nm，最大发射光波长为520～530nm，呈现明亮的黄绿色荧光。（biorbyt也可提供FITC染料，货号：orb1308303）。Alexa Fluor 488：Alexa Fluor 488是一种明亮的绿色荧光染料，其激发设计用于488 nm激光线。Alexa Fluor 488染料在很宽的摩尔范围内对pH不敏感。是免疫荧光和流式细胞术常用的荧光标记物。CY3：Sulfo-Cyanine3 是传统的蛋白质和核酸的橙色荧光标记物(λex=554, λem=568)。CAS 146368-13-0。具有高光稳定性、高抗光漂白性、荧光亮度高、溶于水和有机溶剂以及兼容性好等优点（biorbyt也可提供CY3货号       orb1307215）。PE：藻红蛋白(P-phycoerythrin,PE)是从红藻中分离纯化的，能发出强烈的荧光，具有很好的吸光性能和很高的量子产率，在可见光谱区有很宽的激发及发射范围。是一种有效的荧光探针含有四个携带发色团的亚基，显示出极其明亮的红橙色荧光。(λex=496 nm，λem=578 nm)。CY5：Sulfo-Cyanine5，CY为花菁 (Cyanine) 的缩写，是由奇数个甲基单位连接的两个氮原子组成的化合物。菁类化合物具有波长长、吸收和发射可调、消光系数高、水溶性好、合成相对简单等特点。是一种明亮的红色荧光染料。由于它的激发和发射峰都在长波长红光区，而在这个区域生物样品的自身荧光较弱，因此，相对于Cy3，Cy5具有明显的灵敏度优势。另外，它的荧光（ex：667nm）处在大多数仪器的检测范围内，所以它也是非常常见的荧光标记化合物。（biorbyt也可提供CY5，货号 orb1707415）。PE-CY5：是一种复合荧光染料/串联染料，属于藻红蛋白偶联物，具有吸光性好和光量子产率高的特点，在此类复合染料中，激发能量可从PE传递到Cy5上， 其中PE与Cy5偶联。当被488纳米光激发时，被激发的荧光染料（PE）能够将其荧光能量转移到花菁分子上，然后花菁分子在670纳米处发出更长波长的红色荧光。APC：别藻蓝蛋白（Allophycocyanin）是一种从红藻中分离出的强烈明亮的藻胆蛋白，具有高量子产率的远红色荧光。它被594和633 nm的激光线激发，在650 nm处具有最大吸光度，在660 nm处具有荧光发射峰。直标一抗如何选择？免疫荧光时，通常需要同时检测组织或细胞样本中的多个靶标，常常会利用直标一抗进行免疫荧光共染色，这时一般尽量选择光谱重叠比较少的荧光基团进行组合。例如，可选择一个FITC的直标一抗和 一个AF647的直标一抗进行两个不同蛋白的共染色。根据目标蛋白的表达丰度，选择匹配的荧光标记物，来保证合适的荧光强度。不同荧光标记在不同的仪器上可能强度不同，在某些仪器中：PE>APC>PE-Cy5> FITC。通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原。在流式细胞术时，因为每个抗体只能检测一种荧光，各个通道间的荧光可以随意搭配，例如要同时检测三个靶标，则可在红，绿，黄中各选一个适当的荧光一抗，如PE-CY5, FITC，PE。切忌所有指标选择同一个通道的荧光标记，以防止荧光的重叠和相互干扰。关于流式细胞技术中如何选择荧光标记物，可以点击以下文章链接获取更多详情：【Biorbyt 技术】如何选择合适的流式抗体？Biorbyt 可以提供各种研究领域蛋白指标的标记抗体，以下是近期热销产品列表，欢迎咨询！Catalog  No.NameApplicationsorb475171Avi-Tag  antibody (HRP)WB,  ELISAorb447089TTX(5E7)  antibody (Biotin)ELISAorb448258CRP(C11F2)  antibody (Biotin)WBorb452752Bcl-2  antibody (Biotin)WB,  ELISAorb452942MCherry-Tag  antibody (Biotin)WBorb453007AD7c-NTP 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 antibody (FITC)FC,  IForb102500EDIL3  antibody (FITC)FCorb102575FAPA  antibody (FITC)FC,  IForb102602Filaggrin  antibody (FITC)IForb102620Frizzled  1 antibody (FITC)FC,  IForb102715HDLBP  antibody (FITC)FCorb102779ICAM4  antibody (FITC)FC,  IForb102965MAOA  antibody (FITC)IForb103060Myosin  VIIa antibody (FITC)FC,  IForb103138Nucleoporin  p62 antibody (FITC)FC,  IForb103247Plzf  antibody (FITC)FC,  IForb103461Sema3C  antibody (FITC)IForb103628TNMD  antibody (FITC)IForb103715WASF2  antibody (FITC)IForb3256ZNRF3  antibody (FITC)ICC,  IForb3579Luciferase  antibody (FITC)ICC,  IForb3644Neutrophil  Elastase antibody (FITC)FCorb3898CD71  antibody (FITC)FCorb3910pTrkA  antibody (FITC)FC,  IForb4959CRMP5  antibody (FITC)IForb445057TBR2/Eomes  antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb445157Lubricin  antibody (Biotin)WBorb445402BMP10  antibody (Biotin)ELISAorb446224BRRN1  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb446428Noggin  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb446697Nectin  3 antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb446928CYP2E1  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb446947HOXA5  antibody (Biotin)WBorb447512Insulin  (pig) antibody (Biotin)WBorb447693Syndecan  2 antibody (Biotin)WB,  ELISAorb448001CD40L  antibody (Biotin)IHC-Fr,  IHC-P, ELISAorb448302CNPase  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb448433SOX21  antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb448446Glucose  6 Phosphate Dehydrogenase antibody (Biotin)WB,  ELISAorb448854Lymphotoxin  Beta antibody (Biotin)WB,  ELISAorb449135gp130  (Phospho-Ser782) antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb449632CEP192  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb449677IL24  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb449737FHL1  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb449812CYP8B1  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb450456Influenza  A Nuclear export protein antibody (Biotin)WBorb450868OTOP2  antibody (Biotin)IHCorb451098hydroxyproline  antibody (Biotin)IHC-Fr,  IHC-P, ELISAorb451223LIPT1  antibody (Biotin)ICC,  IHC-Fr, IHC-Porb452158TICAM2  antibody (Biotin)IHCorb452513ABCC9  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-Porb453173TRPV1  antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb453174TRPV1  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-Porb453175Enfuvirtide  antibody (Biotin)ELISAorb453424MALAT1  antibody (Biotin)ELISAorb453551Alpha  Sarcoglycan antibody (Biotin)ICC,  IHC-Fr, WB, IHC-Porb453678SLC12A8  antibody (Biotin)WBorb453735Sec14L3  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb453766LC3A/B  + LC3B + LC3C antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb453822PCDH7  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb454110PDGFRA  antibody (Biotin)IHC-Fr,  WB, ELISAorb499146FCGRT  antibody (PE)ICC,  FC, IForb499317Dopamine  D2 receptor antibody (PE)FC,  IForb499387ECP  antibody (PE)ICCorb499522CD206  antibody (PE)FCorb499560Cryopyrin  antibody (PE)FC,  IForb1152152phospho-Tau  (Thr217) antibodyELISAorb1152153phospho-Tau  (Thr217) antibodyELISAorb501571Bim  (phospho-Ser87) antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb501654BNIP3  (Phospho-Ser95) antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb502213PERK  (phospho-Thr980) antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb502238SGK1  (phospho-Ser78) antibody (Biotin)WBorb502300AKT2  (phospho-Ser474) antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb502577MET  antibody (Biotin)WBorb502710DRP1  (phospho-Ser616) antibody (FITC)ICC,  FC, IForb502961pTau  protein antibody (HRP)ICC,  WB, IHC-P, ELISAorb503318Rb  (phospho-Thr821) antibody (HRP)ICC,  IHC-Fr, WB, IHC-P, ELISAorb503360MLK3  (phospho-Thr277/Ser281) antibody (HRP)IHC-Fr,  IHC-P, ELISAorb503770RASGRP3  (phospho-Thr133) antibody (HRP)ICC,  IHC-Fr, IHC-P, ELISAorb504819p70  S6 Kinase Beta (phospho-Thr389) antibody (PE)FCorb505121BNIP3  (Phospho-Ser95) antibody (PE)FCorb505547EGFR  (phospho-Tyr1068) antibody (PE)ICC,  FC, IForb505631FoxO1  (phospho-Ser256) antibody (PE)FC,  IForb505705PRAS40  (phospho-Thr246) antibody (PE)IForb505720REV-ERB  alpha (phospho-Ser55/59) antibody (PE)FCorb505746Tyk2  (phospho-Tyr1054/1055) antibody (PE)IForb505918FAK  (phospho-Tyr925) antibody (PE)FC,  IForb505973RUNX2  (phospho-Ser451) antibody (PE)FCorb155807BCL2  (Phospho-Ser87) antibodyFC,  IHC-Porb1184460CD69  antibody (FITC)FCorb157497HER3  (Phospho-Tyr1276) antibodyIHC-Porb1184523IL-4  antibody (APC/Cy7)FCorb484247NPHS2  antibody (PE)IForb484364Frizzled  1 antibody (PE)FC,  IForb484431CADM1  antibody (PE)IForb484458Frizzled  5 antibody (PE)FC,  IForb484899PDE5A  antibody (PE)FC,  IForb484959HNF4  antibody (PE)IForb485167CD170  antibody (PE)FC,  IForb485179CLEC2D  antibody (PE)FC,  IForb485226Histone  2AX antibody (PE)ICC,  FC, IForb485352Uteroglobin  antibody (PE)IForb485585MC1  Receptor antibody (PE)FC,  IForb485652PDX1  antibody (PE)orb485741MADH7  antibody (PE)ICC,  FC, IForb485757Synaptophysin  antibody (PE)FC,  IForb485787PDGF  A antibody (PE)FC,  IForb485798Substance  P Receptor antibody (PE)FC,  IForb485838IL1  beta antibody (PE)FC,  IForb485900FGFR2  antibody (PE)ICC,  FC, IForb485929Desmin  antibody (PE)FCorb485949CD10  antibody (PE)ICC,  FC, IForb485955CD20  antibody (PE)FCorb485978GLUT1  antibody (PE)FC,  IForb486695FCER1G  antibody (PE)ICC,  IForb486727Furin  antibody (PE)ICC,  IForb487028GPR177  antibody (PE)ICC,  IForb487111TRPV6  antibody (PE)ICC,  FC, IForb487192TRPV5  antibody (PE)ICC,  FC, IForb487237Calcitonin  receptor antibody (PE)FCorb487601Gasdermin  D antibody (PE)ICC,  IForb488317Claudin  14 antibody (PE)ICC,  IForb488732MSGN1  antibody (PE)FCorb489629Olfactory  Marker Protein antibody (PE)ICC,  IForb489818PCBP1  antibody (PE)ICC,  IForb490178ALPPL2  antibody (PE)ICC,  FC, IForb490198FBP1  antibody (PE)ICC,  IForb490562MET  (c Met) antibody (PE)IForb490863NG2  antibody (PE)FCorb491176SOCS3  antibody (PE)FC,  IForb491183Nurr1  antibody (PE)FC,  IForb491286BTN2A1  antibody (PE)ICC,  FC, IForb491300Synaptopodin  antibody (PE)FCorb491535PCDH7  antibody (PE)ICC,  FC, IForb491891SH2D1B  antibody (PE)ICC,  IForb491900SEZ6  antibody (PE)ICC,  IForb492352METRNL  antibody (PE)FCorb493558FAM105B  antibody (PE)FCorb493841Galectin-1  antibody (PE)FC,  IForb493916LPA2  antibody (PE)ICC,  FC, IForb494692ACAA1  antibody (PE)ICC,  IForb494806Fragilis/IP15  antibody (PE)ICC,  FC, IForb494893SLC38A2/SNAT2  antibody (PE)IForb495013METRN  antibody (PE)FCorb4950755-Lipoxygenase  antibody (PE)FC,  IForb495188GFAP  antibody (PE)FC,  IForb495270p21  antibody (PE)ICC,  FC, IForb495275Vip  Receptor II antibody (PE)FC,  IForb495322STAT5  antibody (PE)ICC,  FC, IForb495392CCR7  antibody (PE)ICC,  FC, IForb495404ZO-1  antibody (PE)FC,  IForb495416NRF1  antibody (PE)ICC,  FC, IForb495426Granzyme  B antibody (PE)FC,  IForb495512CD33  antibody (PE)FCorb495598FOXJ1  antibody (PE)FC,  IForb495625MKP-2/DUSP4  antibody (PE)FC,  IForb495751GLUT12  antibody (PE)ICCorb495793CK19  antibody (PE)FC,  IForb495861Myosin  light chain antibody (PE)FCorb496028Amphiregulin  antibody (PE)IForb496312Neuroligin  1 antibody (PE)FCorb496576SQSTM1  antibody (PE)ICC,  IForb496784Biglycan  antibody (PE)FCorb497141RAG1  antibody (PE)FC,  IForb497495ZNRF3  antibody (PE)ICC,  IForb498438OLIG2  antibody (PE)ICC,  IForb498451SOX4  antibody (PE)ICC,  FC, IForb499007SULF1  antibody (PE)FC,  IForb499027Rabbit  Anti-Sca1 (PE)FC,  IForb499038Versican  antibody (PE)orb499041HER2  antibody (PE)FC,  IForb499090PDGF  B antibody (PE)FC,  IForb499099Mesothelin  antibody (PE)FCorb499101N  Cadherin antibody (PE)ICC,  FC, IForb110119Cox2  antibody (HRP)IHC-Fr,  WB, IHC-P, ELISAorb110148DKK1  antibody (HRP)IHC-Fr,  IHC-P, ELISAorb110168Envelope  Glycoprotein Yellow Fever Virus antibody (HRP)WBorb110181FGF23  antibody (HRP)IHC-Fr,  WB, IHC-P, ELISAorb110200GLP1  antibody (HRP)IHC-Fr,  IHC-P, ELISAorb110280Klotho  antibody (HRP)IHC-Fr,  WB, IHC-P, ELISAorb463033SLC34A1  antibody (FITC)ICC,  IForb463034SLC34A3  antibody (FITC)FCorb463588IL-1R1  antibody (FITC)ICC,  FC, IForb463625CD19  antibody (FITC)FCorb463970Caveolin-3  antibody (FITC)ICC,  IForb464231Nurr1  antibody (FITC)FC,  IForb464334BTN2A1  antibody (FITC)ICC,  FC, IForb464413PCDH7  antibody (FITC)ICC,  FC, IForb464643SLC4A8  antibody (FITC)ICC,  IForb464701RGS13  antibody (FITC)ICC,  IForb464764RASGRP2  antibody (FITC)ICC,  IForb464939Claudin  16 antibody (FITC)ICC,  IForb464951CD200R  antibody (FITC)ICC,  FC, IForb464992DAP12  antibody (FITC)FC,  IForb465963CD147  antibody (FITC)FC,  IForb466005MADH7  antibody (FITC)ICC,  FC, IForb466068IL1  Receptor 2 antibody (FITC)FC,  IForb466253ET-2/Endothelin  2 antibody (FITC)ICC,  IForb466729EN1/Engrailed  1 antibody (FITC)ICC,  FC, IForb466823GRIN3A  antibody (FITC)ICC,  IForb466849iNOS  antibody (FITC)ICC,  FC, IForb466860CD11c  antibody (FITC)FCorb466967beta  1 Adrenergic Receptor antibody (FITC)FC,  IForb467157SOSSC  antibody (HRP)WBorb468610PPIA  antibody (HRP)IHC-Fr,  WB, IHC-P, ELISAorb468629Endomucin  antibody (HRP)ICC,  IHC-Fr, WB, IHC-P, ELISAorb468766ENaCg  antibody (HRP)WB,  IHC-P, ELISAorb110441TGF  beta Receptor I (HRP)IHC-Fr,  WB, IHC-P, ELISAorb110442TGF  beta Receptor 2 antibody (HRP)IHC-Fr,  WB, IHC-P, ELISAorb11392514-3-3  antibody (Biotin)WBorb114121FGF23  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb114190IL6  alpha Receptor antibody (Biotin)IHC-Fr,  IHC-P, ELISAorb114226LC3A/B  antibody (Biotin)IHC-Fr,  IHC-P, ELISAorb114301OxLDL  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb124537CD69  antibody (PE)FC,  IForb124560Cox2  antibody (PE)FC,  IForb124566CXCL10  antibody (PE)FC,  IForb124590DKK3  antibody (PE)IForb124596EAR1  antibody (PE)FC,  IForb124613EPOR  antibody (PE)IForb124697iNOS  antibody (PE)IForb124701Integrin  alpha 5 beta 3 antibody (PE)FC,  IForb124805PAF  Receptor antibody (PE)FC,  IForb124889Tissue  Factor antibody (PE)IForb454412MX1  antibody (Biotin)WB,  ELISAorb454440Nesprin  3 antibody (Biotin)ICC,  IHC-Fr, WB, IHC-Porb454825LTBP2  antibody (Biotin)WBorb455080MRP6  antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb455495KREMEN2  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb455837FAM208B  antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb456376CLEC9A  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb456579C8orf42  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb457174Spinophilin/Neurabin  2 antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb457307METRN  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb457443KGF  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb457605TTF1  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-Porb457949LPL  antibody (Biotin)WBorb458045IL31  Receptor alpha antibody (Biotin)WB,  IHCorb458068OX40  antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb458103Brucella  antibody (Biotin)ELISAorb458315ROR  Gamma antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb458401ACADVL  antibody (Biotin)IHC-Fr,  IHC-P, ELISAorb458538SOX9  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb458592CDKN2B  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb458681Alpha  B Crystallin antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb458821RAB5  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb459585SARM1  antibody (Biotin)IHC-Fr,  IHC-P, ELISAorb459776TDRD9  antibody (Biotin)IHC-Fr,  ELISAorb459893BST2  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb459958GABR  B3 antibody (Biotin)WBorb459963HOPX  antibody (Biotin)IHC-Fr,  IHC-P, ELISAorb460116Transglutaminase  2 antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb460181LHX2  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb460707Exendin  4 antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb460826GRIN3A  antibody (Biotin)ICC,  IHC-Fr, WB, IHC-P, ELISAorb461054CGRP  antibody (Biotin)ICC,  IHC-Fr, IHC-P, ELISAorb461071ACTA2  antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISAorb461103HCV  antibody (Biotin)ELISAorb461433Cathepsin  B antibody (FITC)ICC,  FC, IForb461506Nogo  A antibody (FITC)FC,  IForb461618IGF1R  antibody (FITC)ICC,  FC, IForb462107DMC1  antibody (FITC)ICC,  IForb462143ESAT6  antibody (FITC)ICC,  IForb462375GPR110  antibody (FITC)ICC,  FC, IForb462503GPCR  EX33 antibody (FITC)ICC,  FC, IForb462617TRPV6  antibody (FITC)ICC,  FC, IForb462681PLAC8  antibody (FITC)FCorb190914ZNF683  antibody (FITC)ICC,  IForb222130MDR1  antibody (FITC)ICC,  FC, IForb222371Nephronectin  antibody (FITC)ICC,  IForb544636TWIST  antibody (FITC)IForb572747Goat  Anti-Rabbit IgG H&L antibody (HRP)IHC-P,  ELISAorb654253Mongolian  Gerbil IgG antibody (Biotin)IHC-Fr,  WB, IHC-P, ELISA, IForb868194Rabbit  Anti-MG IgG H&L antibody (HRP)IHC-Fr,  WB, IHC-P, ELISAorb868396Mouse  Anti-Human IgM antibody (Cy5)FCorb868531Goat  Anti-Rat IgG H&L antibody (Cy5)FCorb868595Goat  Anti-Rabbit IgG H&L antibody (PE-Cy5)FCorb868754Donkey  Anti-Guinea Pig IgG H&L antibody (Cy3)IForb868831Rabbit  Anti-Bovine IgM antibody (FITC)FCorb871516Anti-IL-1  Beta/PE-Cy5FCorb875283Anti-APR3/APIHC-Fr,  IHC-P, ELISAorb878042Anti-MARCH9/PE-Cy7FCorb879446Anti-IRAK1/PE-Cy7ICC,  FCorb880553Anti-IFNAR1/PE-Cy5ICCorb880682Anti-phospho-HNF4  (Ser304)/PE-Cy7FCorb881904Anti-PPAR  Gamma/PE-Cy5.5FCorb889795Anti-PRSS8/APIHC-Fr,  WB, IHC-P, ELISAorb891238Anti-FAPA/Cy5FC,  IForb891331Anti-Neuritin/PE-Cy5FCorb891519Anti-Polycystin  1/PE-Cy5ICC,  FCorb892191Anti-Ferritin  Heavy Chain/FTH1/PE-Cy7ICC,  FCorb893857Anti-phospho-TrkB  (Tyr705)/APICC,  IHC-Fr, WB, IHC-Porb894408Anti-PP1C  gamma/APIHC-Fr,  WB, IHC-P, ELISAorb895734Anti-PKM2/PE-Cy7ICCorb899278CD11b/PE-Cy5  antibodyFCorb899559Anti-Caveolin-3/Cy5FC,  IForb899751Anti-PXR/PE-Cy5.5FCorb901017Anti-CD1d/PE-Cy5.5FCorb902613Phospho-PERK(Thr980)/PE-Cy7  antibodyFCorb905794Anti-CD170/PE-Cy5.5FCorb909518Anti-CCR10/PE-Cy7FCorb909815Anti-Thioredoxin/Cy5ICC,  FC, IForb910328Anti-FAK/APIHC-Fr,  WB, IHC-P, ELISAorb910370Profilin  1/PE-Cy5.5 antibodyFCorb910865Anti-Casein/APWBorb911309Anti-BRG-1/PE-Cy7FCorb913013Anti-KRAS/Cy5FC,  IForb913028Anti-IL12/PE-Cy5.5FCorb913704Anti-BLBP/Cy5FC,  IForb913879Anti-GFAP/Cy5FC,  IForb914484Anti-Nestin/PE-Cy7ICC,  FCorb915495Anti-CD19/Cy5FC,  IForb915599Anti-alpha  smooth muscle Actin/PE-Cy3FCorb917495Anti-F-Actin/PE-Cy7FCorb917499Anti-RAGE/PE-Cy7FCorb917649Anti-GLUT1/PE-Cy5.5FCorb926868Anti-IP3  receptor/PE-Cy7ICCorb928016Anti-GPR89A/APWBorb939512Anti-FLJ23834/PE-Cy7ICCorb941999Anti-NeuN/PE-Cy7ICC,  FCorb958664Anti-MX1/APWB,  ELISAorb959894Anti-GFAP/PE-Cy5.5ICC,  FCorb959955Anti-NFKB  p65(acetyl-K310)/PE-Cy5FCorb967450Phospho-Smad2  (Ser250)/PE-Cy5.5FCorb967820Anti-VWF/PE-Cy7FCorb967825Anti-Synaptopodin/PE-Cy7FCorb970948Anti-CD80/Cy5IForb972119Anti-PLVAP/Cy5ICC,  IForb973383Anti-PXR/PE-Cy5FCorb979674Anti-ZNF683/Cy3FCorb982588Anti-Sca1/Cy3FC,  IForb986438Anti-CD36/Cy3IForb991834Anti-SP-C/APCFCorb993436Anti-Bone  Alkaline Phosphatase/APCFC,  IForb993553Anti-CD16/APCFC,  IForb993602Anti-SET/APCICCorb993611Anti-Calreticulin/APCFC,  IForb994742Anti-SREBP2/APCICC,  FC, IForb994938Anti-Dopamine  D5 receptor/APCFC,  IForb995017Anti-Claudin  1/APCFCorb995081Anti-CXCL14/APCIForb995082Anti-CXCL10/IP10/APCFC,  IForb995560Anti-CD20/APCFCorb997261Anti-Ephrin  A4/APCICC,  IForb998000Anti-SPACA1/APCICC,  IForb998027Anti-CD44v3/APCICC,  FC, IForb1000842Anti-Phospho-MYPT1(Thr696)/APCICC,  FC, IForb1001893Anti-VNN1/APCICC,  FC, IForb1004511Anti-CD36/APCIForb1004685Anti-FCGRT/APCICC,  FC, IForb1005088Anti-Fragilis/APCICC,  FC, IForb1005524Anti-Cytokeratin  4/APCFC,  IForb1005708Anti-Nogo-A/APCFC,  IForb1005735Anti-PDGFRA/APCFC,  IForb1005736Anti-PDGF-B/APCFC,  IForb1005799Anti-TGF  beta Receptor I/APCFC,  IForb1006424Anti-EGFRvIII/APCICC,  FC, IForb1006496Anti-CLEC2D/APCFC,  IForb1007330Anti-Phospho-mTOR  (Thr2446) /APCFC,  IForb1007509Anti-SOX9/APCFC,  IForb1007931Anti-CKAP4/APCFC,  IForb1009483Anti-PAX6/APCICC,  FC, IForb1009572Anti-TPOR/APCFCorb1046500Anti-Amphiregulin/Cy5.5FCorb1048324Anti-Phospho-Histone  H2A.X (Ser139)/Cy7ICC,  FC, IForb1597031Procalcitonin  (PCT) Antibody (Biotin)WB,  ELISAorb1597253mScarlet  Antibody (AP)WBorb1602436S100A8  Antibody (Biotin)IHC-Porb1611053Rabbit  IgG / PE-Cy7 Antibodyorb1727929Recombinant  human CD63 protein,  hFc (HEK293)orb1572134Phospho-HSD17B13  (Ser33) Antibody (HRP)ICC,  WBorb1577230VEGFR3  Antibody (Cy5)ICC,  FCorb1581210IFNAR1  Antibody (PerCP)ICCorb1583206E  cadherin Antibody (PerCP)ICC,  FCorb1584383RUNX1  Antibody (BF555)FCorb1588962ganglioside  GM1 Antibody (PerCP)FCorb158995514-3-3  Antibody (BF750)FCorb158995614-3-3  Antibody (BF680)FCorb158995714-3-3  Antibody (BF647)FCorb158995814-3-3  Antibody (BF594)FCorb158995914-3-3  Antibody (BF555)FCorb158996014-3-3  Antibody (BF488)FCorb158996114-3-3  Antibody (BF405)FCorb158996214-3-3  Antibody (BF350)FCorb158996314-3-3  Antibody (Cy7)FCorb158996414-3-3  Antibody (Cy5.5)FCorb158996514-3-3  Antibody (PerCP)FCorb158996614-3-3  Antibody (Cy7)FCorb158996814-3-3  Antibody (Cy5)FCorb1592229Phospho-GCN2  (Thr899) Antibody (Cy5)FCorb1594135GLUT1  Antibody (BF555)FC,  IForb1594414Annexin  V Antibody (Cy5.5)FCorb1594732HSP60  Antibody (Cy7)ICCorb1595292LPAP  Antibody (PerCP)FCorb1595912FOXM1  Antibody (HRP)WBorb1597136ASFV  p30 Antibody (HRP)WBorb1598709Tap1  Antibody (BF405)FC,  IForb1599471ASFV  p72 Antibody (HRP)WB,  ELISAorb1600370VEGFA  Antibody (BF488)FC,  IForb1600770IL-15  Antibody (BF405)ICC,  FC, IForb1600788CREM  Antibody (PerCP)ICC,  FCorb1601401PIEZO1  Antibody (BF488)ICC,  FC, IForb1602128Melatonin  Receptor 1A Antibody (Cy7)FCorb1603027PODXL  Antibody (BF555)FC,  IForb1606712phospho-IKK  beta (Tyr188) Antibody (Cy5)FCorb1608014TGF  beta Receptor I Antibody (PerCP)FCorb1609870Geminin  Antibody (APC/Cy5)FCorb18160913326-32-7  Antibody (AP)WBorb18160923326-32-7  Antibody (FITC)FCorb18160943326-32-7  Antibody (HRP)WB扫码获得英国biorbyt热销标记抗体的详细产品信息清单 收起