
 
| 价格 | ¥2717.00 | 
| 发货 | 湖北武汉市 | 
| 运费 | 包邮:2000.00 | 
| 规格 | 
 | 
| 数量 | -+ | 
| 库存 | 10件起订1件 | 
https://www.biorbyt.com/human-nedd4-le3-ubiquitin-pro-orb1146830.html
| Catalog Number | orb1146830 | 
|---|---|
| Category | Assays and Kits | 
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E3 ubiquitin-protein ligase NEDD4-like(NEDD4-L). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E3 ubiquitin-protein ligase NEDD4-like(NEDD4-L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E3 ubiquitin-protein ligase NEDD4-like(NEDD4-L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E3 ubiquitin-protein ligase NEDD4-like(NEDD4-L) in the samples is then determined by comparing the OD of the samples to the standard curve. | 
| Reactivity | Human | 
| Range | 0.32-20 ng/mL | 
| UniProt ID | Q96PU5 | 
| Sensitivity | 0.119 ng/mL | 
| Protein Length | 3.5h | 
| Alternative names | NED4L;NEDD4 2 Read less | 
| Note | For research use only | 
| Sample Types | serum, plasma, tissue homogenates and other biological fluids | 
| Expiration Date | Please enquire. |