Rabbit NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit orb1736725
https://www.biorbyt.com/rabbit-nfb-p65nuclear-factor-orb1736725.html
| Catalog Number | orb1736725 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Rabbit |
| Range | 46.88-3000 pg/mL |
| Sensitivity | 18.1 pg/mL |
| Alternative names | NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light P Read more... |
| Note | For research use only |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Expiration Date | Please enquire. |