Rat 5HTR2A(5-Hydroxytryptamine Receptor 2A) ELISA Kit orb1146868
https://www.biorbyt.com/rat-5htr2a5-hydroxytryptamine-orb1146868.html
| Catalog Number | orb1146868 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 2A(5HTR2A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine Receptor 2A(5HTR2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Hydroxytryptamine Receptor 2A(5HTR2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine Receptor 2A(5HTR2A) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Rat |
| Range | 0.16-10 ng/mL |
| UniProt ID | P14842 |
| Sensitivity | 0.065 ng/mL |
| Protein Length | 3.5h |
| Alternative names | 5-HT2A, HTR2, Serotonin Receptor 2A Read less |
| Note | For research use only |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Expiration Date | Please enquire. |