Pig MIP1b ELISA Kit orb1173618
https://www.biorbyt.com/pig-mip1b-elisa-kit-orb1173618.html
| Catalog Number | orb1173618 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Beta(MIP1b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Beta(MIP1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Beta(MIP1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Beta(MIP1b) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Tested applications | ELISA |
| Reactivity | Porcine |
| Range | 78.13-5000 pg/mL |
| Sensitivity | 30 pg/mL |
| Protein Length | 3.5h |
| Alternative names | MIP1 Beta, CCL4, ACT2, G-26, LAG1, MIP1-B, SCYA4, Read more... |
| Note | For research use only |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
| Expiration Date | Please enquire. |