Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit orb1736579
https://www.biorbyt.com/human-anti-ttg-iga-anti-tissu-orb1736579.html
| Catalog Number | orb1736579 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Human |
| Range | 4.69-300 IU/mL |
| Sensitivity | 1.47 IU/mL |
| Alternative names | Anti-Tissue Transglutaminase IgA Read more... |
| Note | For research use only |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids. |
| Expiration Date | Please enquire. |